Research Paper Volume 12, Issue 17 pp 16803—16819

Anchorage independence altered vasculogenic phenotype of melanoma cells through downregulation in aminopeptidase N /syndecan-1/integrin β4 axis

Figure 5. ANPEP regulated SDC1 and integrin β4 expression through PKCδ phosphorylation. (A) Suppression of ANPEP expression by ANPEP-specific shRNAs and were examined by qPCR and flow cytometry. Data were mean ±S.D. (n=3); **, p < 0.01. (B) Effect of ANPEP downregulation at expression of SDC1 and integrin isoforms as examined by qPCR. Data were mean ±S.D. (n=3); **, p < 0.01. (C) Effect of ANPEP downregulation at expression of integrin isoforms, SDC1, and PKCδ phosphorylation as examined by western blot.