Research Paper Volume 12, Issue 14 pp 14219—14231

Decreased lncRNA, TINCR, promotes growth of colorectal carcinoma through upregulating microRNA-31

Figure 5. Suppression of TINCR promotes CRC cell growth in vitro without affecting cell apoptosis. (A, B) The effects of TINCR modification on Caco-2 (A) and SW480 (B) cell growth were examined in vitro by CCK-8 assay. (C, D) Cell apoptosis was examined in Caco-2 cells by Annexin V/PI-based flow cytometry assay, shown by representative flow charts (C) and by quantification (D). (E, F) Cell apoptosis was examined in SW480 cells by Annexin V/PI-based flow cytometry assay, shown by representative flow charts (E) and by quantification (F). N=5. *p<0.05. NS: non-significant.