Figure 2. Reduced tumor growth in mice with C5aR deficiency or C5aR signaling blockade. Mice were received the murine BC cell line 4T-1 cells transplantation for tumor development. Tumor size was measured every 2 days. Mice were sacrificed in the indicated time and tumors tissues were collected. (C5aRa treatment: WT mice were injected with C5aRa 30 min prior to 4T-1 cells transplantation. Saline was used as the control of C5aRa). (A) Size and weight of tumors from WT and C5aR KO mice. (B) Ki67 and CD146 expression in WT and C5aRKO mice. (C) Size and weight of tumors from WT mice pretreated with Saline or C5aRa. (D) Ki67 and CD146 expression in WT mice with C5aRa and saline. (n = 5-6/group). Magnification: 200×. Three independent experiments were performed. *P < 0.05, **P < 0.01, ***P < 0.001.