Research Paper Volume 12, Issue 14 pp 14300—14313

Caveolin-3 protects diabetic hearts from acute myocardial infarction/reperfusion injury through β2AR, cAMP/PKA, and BDNF/TrkB signaling pathways

Figure 4. Caveolin-3 promotes the localization of β2AR onto the cytomembrane of H9C2 cells. (A, B) H9C2 cells were transfected with pcDNA3.1/Caveolin-3 to conduct Caveolin-3 overexpression. The total protein levels of Caveolin-3 and ADRB2 in Caveolin-3-transfected cells were determined using immunoblotting, (n=3). (C, D) The membrane protein levels of ADRB2 in Caveolin-3-transfected cells were determined using immunoblotting, (n=3). (E) The content and distribution of ADRB2 protein in the control, H/R, H/R + HG, and H/R + HG + Caveolin-3 groups determined by IF staining, (n=3). (FH) H9C2 cells were transfected with pcDNA3.1/Caveolin-3 under H/R or HG stimulation and examined for cell apoptosis by Flow cytometry (n=3) and cell viability by MTT assay (n=5). *P<0.05, **P<0.01 compared to pcDNA3.1+H/R group; ##P<0.01, compared to pcDNA3.1+H/R group.