Research Paper Volume 12, Issue 14 pp 14341—14354

LKB1 inactivation leads to centromere defects and genome instability via p53-dependent upregulation of survivin

Figure 1. LKB1 maintains genome stability in U2OS cells. (A) DAPI staining showing accumulation of misaligned chromosomes at metaphase in U2OS/sh-Ctrl and U2OS/sh-LKB1 cells and corresponding data quantification. (B) DAPI staining showing accumulation of lagging chromosomes at anaphase in U2OS/sh-Ctrl and U2OS/sh-LKB1 cells and corresponding data quantification. (C) Quantification of mitotic index based on p-H3 (Ser10) staining. (D) Quantification of multinucleated cells. Cells with 2 or more nuclei were counted. (E) Quantification of micronucleated cells. Chromosome segregation defects were recorded in 100 randomly selected mitoses from each experiment (n=3). Data are mean ± SD; *P<0.05.