Research Paper Volume 12, Issue 14 pp 14341—14354

LKB1 inactivation leads to centromere defects and genome instability via p53-dependent upregulation of survivin

Figure 3. LKB1 improves genome stability in LKB1-deficient A549 cells. (A) DAPI staining showing accumulation of misaligned chromosomes at metaphase in A549 cells and corresponding data quantification. (B) DAPI staining showing accumulation of lagging chromosomes at anaphase in A549 cells and corresponding data quantification. (C) Mitotic index quantification based on p-H3 (Ser10) staining. (D) Quantification of multinucleated cells. (E) Quantification of micronucleated cells. Chromosome segregation defects were recorded in 100 randomly selected mitoses from each experiment (n=3). Data are mean ± SD; *P<0.05.