Research Paper Volume 12, Issue 14 pp 14341—14354

LKB1 inactivation leads to centromere defects and genome instability via p53-dependent upregulation of survivin

Figure 4. LKB1 represses survivin expression. (A) Western blotting detection of survivin and Aurora B in U2OS/sh-Ctrl and U2OS/sh-LKB1 cells. (B) Western blotting detection of survivin and Aurora B in A549/tet (control) and A549/tet-LKB1 cells. (C) Western blotting detection of survivin in Hela cells after induced expression of LKB1. (D) Western blotting detection of survivin in MEF, 293T, and C33A cells after LKB1 knockdown. Thirty micrograms of protein per lane were loaded. GAPDH served as loading control. (E) Immunofluorescence staining showing centromeric localization of survivin in U2OS cells.