Research Paper Volume 12, Issue 17 pp 16820—16836

Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines

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Figure 4. NETs stimulate HBEs and A549 to express neutrophilic chemokines. (A and B) CXCL1, CXCL2, and CXCL8 expression in the supernatant was analyzed by Luminex and RT-qPCR, after HBEs (or A549) were treated with NETs co-cultured for 24 h. (C and D) Neutrophil migration was analyzed by transwell after cell culture supernatant was induced for 48 h (200X). NET treated supernatant was added to the lower chamber. #: P<0.05, ##: P<0.01, ###: P<0.001, ####: P<0.0001 vs control group. *: P<0.05, **: P<0.01, ***: P<0.001, ****: P<0.0001 vs NA group. ns = no statistical difference vs NETs group.