Figure 4. Regulation of miR-15a/16/SOX5 axis on RA-FLSs migration and invasion. (A) Following transfected with miR-15a, miR-16 mimics and miR-control for 48h, FLSs subjected to transwell (A, above) and transwell chamber invasion assay (A, below) after 24h. (B) Following transfected with miR-15a, miR-16 mimics and miR-control for 48h, FLSs were fixed and stained with FITC-phalloidin. Representative confocal microscopy images of three independent experiments are shown to illustrate stress fibers and appearance of lamellipodia. (C, D) Graphs show the quantitation data derived from the left figure A. Data are each representative of 3 independent experiments. (E, F) SOX5 overexpression alleviates the miR-15a/16 mimics-mediated inhibitory roles on migration (E) and invasion (F) in FLSs. Graphs show the quantitation data derived from 3 independent migration and invasion assay.