Research Paper Volume 12, Issue 14 pp 14418—14433

miR-5089-5p suppresses castration-resistant prostate cancer resistance to enzalutamide and metastasis via miR-5089-5p/SPINK1/ MAPK/MMP9 signaling

Figure 3. SPINK1 enhances migration and invasion of Enz-resistant cells via MAPK/MMP9 signaling. (A, B) Migration rates of shSPINK1 C4-2R and C4-2B-R cells, and oeSPINK1 C4-2 andC4-2B cells in the wound healing assay. (C, D) Invasive capacity of shSPINK1 C4-2R and C4-2B-R cells, and oeSPINK1 C4-2 and C4-2B cells in the transwell assay. (E, F) Correlation of SPINK1 expression with MMP9 and Ki67 levels in PCa tissues of TCGA and GSE66187 datasets. (GJ) Relative p-ERK, p-p38, Ki67 and MMP9 protein levels in shSPINK1 C4-2R and C4-2B-R cells, and oeSPINK1 C4-2 and C4-2B cells. Each bar represents the mean ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001.