Research Paper Volume 12, Issue 14 pp 14467—14479

The regulatory role of miR-107 in Coxsackie B3 virus replication

Figure 1. (AB) Hela cells were infected with CVB3 with specified titers at planned time points. Total RNA of the cells were extracted and miR-107 was detected using real-time PCR. (CE) Hela cells were infected at a certain concentration of CVB3, and viral replication at different time points was observed. (F) The transfection efficiency of miR-107 mimics was analyzed using RT-qPCR, and western blot reflected viral replication at a protein level. Viral titers were determined by plaque assay. (G) The transfection efficiency of miR-107 inhibitors was detected by RT-qPCR, and western blot reflected virus replicated at a protein level. Viral titers were determined by plaque assay. (H) The replication level of EGFP-Iabeled CVB3 was observed in Hela cells which had been transfected with mimics107 or 107-in (magnification, 4×).