Research Paper Volume 12, Issue 17 pp 16837—16851

Figure 1. SFN-Cys decreased the expressions of CDK4/CDK6 and TUBA1C. TUBA1C mRNA expression showed positive correlation to pathological grades and negative correlation to clinical prognosis in glioma. The deletion rates of CDKN2A and CDKN2B were higher in glioma patients. (A) After U87MG cells were treated with or without SFN-Cys for 24 h, the extracted proteins were analyzed for expression; protein expressions and Ontology were analyzed via HPLC-MS/MS and the Uniprot net. (B) Differential expressions of mRNA were analyzed in normal and tumor tissues via GEPIA database, which matched TCGA normal and GTEx data [46]. LGG: Low grade glilma. (C, D) The deletion rates of CDKN2A and CDKN2B were analyzed by means of database of Glioblastoma Multiforme (TCGA, Provisional) and of Merged Cohort of LGG and GBM (TCGA, Cell 2016) via cBioPortal for Cancer Genomics. (E) The expression and survival analysis of TUBA1C mRNA in glioma patients were done via GEPIA Database, which matched TCGA normal and GTEx data. (F–H). The expression and survival analysis of TUBA1C mRNA in glioma patients with primary and recurrent were done via CGGA database. Data were shown as *P < 0.05; **P < 0.01; ***P < 0.001, NS no significance vs. control group, n ≥ 3.