Research Paper Volume 12, Issue 18 pp 17990—18007
SIRT1 activation by minocycline on regulation of microglial polarization homeostasis
Figure 8. Effects of minocycline on phagocytosis in microglia. Phagocytosis of 1-μm fluorescent YG beads by IMG and BV-2 microglia was analyzed by flow cytometry. (A, C) Cells with or without minocycline treatment (20 μM), or (B, D) those pretreated with minocycline for 30 min before LPS stimulation for 24 h, were incubated with the YG beads for 1 h at 37°C before flow cytometry analysis. Each graph represents data from at least 3 biological replicates.