Research Paper Volume 12, Issue 14 pp 14808—14818

Hsa_circ_0044226 knockdown attenuates progression of pulmonary fibrosis by inhibiting CDC27

Figure 3. Hsa_circ_0044226 knockdown suppresses TGF-β1-induced RLE-6TN cell migration. (A) qRT-PCR analysis of hsa_circ_0044226 levels in RLE-6TN cells infected with a lentiviral vector encoding hsa_circ_0044226 shRNA1 or hsa_circ_0044226 shRNA2. (B) RLE-6TN cells were pretreated with 10 ng/mL TGF-β1 for 48 h and then transformed with hsa_circ_0044226 shRNA2 for 0, 24, 48 or 72 h. CCK-8 assays were applied to determine the cell viability. (C, D) RLE-6TN cells were pretreated with 10 ng/mL TGF-β1 for 48 h and then transformed with hsa_circ_0044226 shRNA2 for 72 h. Relative fluorescences were quantified with Ki67 and DAPI staining. (E, F) Cell migration was detected using wound healing assays. **P < 0.01 vs. Blank group. ##P < 0.01 vs. TGF-β1 group.