Research Paper Volume 12, Issue 14 pp 14897—14917

Neuroprotective and neurogenic effects of novel tetramethylpyrazine derivative T-006 in Parkinson’s disease models through activating the MEF2-PGC1α and BDNF/CREB pathways

Figure 7. T-006 enhances neural reconstruction by stimulating neurogenesis in rat model of PD. Immunofluorescence images of ipsilateral hemisphere sections co-stained with antibodies against (A) TH (green, a marker of DA neurons) and BrdU (red, a marker of proliferating cells); (C) DCX (green, a marker of migrating neuroblasts) and BrdU (red); (E) Nestin (green, a marker of NPCs) and BrdU (red) cells. DAPI (blue) indicates nucleus. Insets show a higher magnification view of double-positive cells. Scale bar = 200 μm for whole slice, 50 μm for inset magnification. (B, D and F) Quantitative analysis of newly formed mature neurons (BrdU+/TH+, D), in the SN, migrating neuroblasts (BrdU+/DCX+, E) and proliferating NPCs (BrdU+/Nestin+, F) in the SVZ. (G and I) Immunohistochemical images of ipsilateral hemisphere sections co-stained with antibodies against DCX and Nestin in the SNc. (H and J) Quantitative analysis of DCX and Nestin-positive cells in SNc. Data are expressed as mean±SEM (n=3 to 4 per group). *P<0.05, **P<0.01 and ***P<0.001 vs. 6-OHDA group.