Research Paper Volume 13, Issue 10 pp 14456—14468

Cancer-associated fibroblasts secreted miR-103a-3p suppresses apoptosis and promotes cisplatin resistance in non-small cell lung cancer

Pum2 promoted exosomal miR-103a-3p secretion and cisplatin resistance in NSCLC cells. (A) qRT-PCR analyzed the expression of miR-103a-3p NCI-H1650 and NCI-H1299 cells incubated with CAFs transfected Pum2 plasmid with or without AMO-miR-103a-3p. (B) qRT-PCR analyzed the expression of miR-103a-3p NCI-H1650 and NCI-H1299 cells incubated with CAFs transfected si-Pum2 with or without miR-103a-3p. (C, D) CCK8 was used to test viability of NCI-H1650 and NCI-H1299 cells. (E, F) The expressions of apoptosis related protein Bax, Caspase3 and Caspase9 were analyzed by western bolt. (G, H) The number of apoptotic cells was calculated by flow cytometry. *p# p

Figure 4. Pum2 promoted exosomal miR-103a-3p secretion and cisplatin resistance in NSCLC cells. (A) qRT-PCR analyzed the expression of miR-103a-3p NCI-H1650 and NCI-H1299 cells incubated with CAFs transfected Pum2 plasmid with or without AMO-miR-103a-3p. (B) qRT-PCR analyzed the expression of miR-103a-3p NCI-H1650 and NCI-H1299 cells incubated with CAFs transfected si-Pum2 with or without miR-103a-3p. (C, D) CCK8 was used to test viability of NCI-H1650 and NCI-H1299 cells. (E, F) The expressions of apoptosis related protein Bax, Caspase3 and Caspase9 were analyzed by western bolt. (G, H) The number of apoptotic cells was calculated by flow cytometry. *p<0.05 vs CAF-NC exo or CAF-si-NC exo, # p<0.05 vs CAF-Pum2 exo+AMO-miR-103a-3p or CAF-si-Pum2 exo+miR-103a-3p.