Research Paper Volume 13, Issue 10 pp 14456—14468

Cancer-associated fibroblasts secreted miR-103a-3p suppresses apoptosis and promotes cisplatin resistance in non-small cell lung cancer

Bak1 was a direct target of exosomal miR-103a-3p in NSCLC cells. (A) Predicted miR-103a-3p target sequences in the 3′ UTRs of Bak1 genes. (B) Bak1 mRNA expression in NCI-H1650 and NCI-H1299 transfected with miR-103a-3p or AMO- miR-103a-3p at 48 h after transfection. (C) Bak1 mRNA levels in NCI-H1650 and NCI-H1299 cells at 48 h after incubation with CAF exosomes. (D) WT and mutant Bak1 luciferase plasmids were transfected into HEK293 cells with miR-103a-3p or AMO- miR-103a-3p. The luciferase activity was measured by dual-luciferase reporter assay system. (E) The effects of CAF exosomes on Bak1 reporter luciferase activity in HEK293 cells. *p

Figure 5. Bak1 was a direct target of exosomal miR-103a-3p in NSCLC cells. (A) Predicted miR-103a-3p target sequences in the 3′ UTRs of Bak1 genes. (B) Bak1 mRNA expression in NCI-H1650 and NCI-H1299 transfected with miR-103a-3p or AMO- miR-103a-3p at 48 h after transfection. (C) Bak1 mRNA levels in NCI-H1650 and NCI-H1299 cells at 48 h after incubation with CAF exosomes. (D) WT and mutant Bak1 luciferase plasmids were transfected into HEK293 cells with miR-103a-3p or AMO- miR-103a-3p. The luciferase activity was measured by dual-luciferase reporter assay system. (E) The effects of CAF exosomes on Bak1 reporter luciferase activity in HEK293 cells. *p<0.05 vs miR-NC or AMO-miR-NC or NF exo.