Research Paper Volume 13, Issue 10 pp 14456—14468

Cancer-associated fibroblasts secreted miR-103a-3p suppresses apoptosis and promotes cisplatin resistance in non-small cell lung cancer

miR-103a-3p promotes cisplatin resistance in NSCLC cells by inhibiting Bak1. NCI-H1650 and NCI-H1299 cells were transfected with Bak1 or si- Bak1 and incubated with exosomes from CAFs transfected with miR-103a-3p or AMO-103a-3p, respectively. (A, B) CCK8 was used to test viability of NCI-H1650 and NCI-H1299 cells. (C, D) The expressions of apoptosis related protein Bax, Caspase3 and Caspase9 were analyzed by western bolt. (E, F) The number of apoptotic cells was calculated by flow cytometry. *p

Figure 6. miR-103a-3p promotes cisplatin resistance in NSCLC cells by inhibiting Bak1. NCI-H1650 and NCI-H1299 cells were transfected with Bak1 or si- Bak1 and incubated with exosomes from CAFs transfected with miR-103a-3p or AMO-103a-3p, respectively. (A, B) CCK8 was used to test viability of NCI-H1650 and NCI-H1299 cells. (C, D) The expressions of apoptosis related protein Bax, Caspase3 and Caspase9 were analyzed by western bolt. (E, F) The number of apoptotic cells was calculated by flow cytometry. *p<0.05 vs CAF-miR-103a-3p exo+NC or CAF-AMO-miR-103a-3p exo+si-NC.