Figure 2. Reduction in NR2F1-AS1 expression results in attenuated proliferation, migration, and invasion as well as promoted apoptosis of H460 and A549 cells. (A) H460 and A549 cells were transfected with three different siRNAs targeting NR2F1-AS1 and si-NC, and the level of NR2F1-AS1 was determined by RT–qPCR. (B) CCK-8 assay demonstrated that silencing of NR2F1-AS1 suppressed the proliferative potential of H460 and A549 cells. (C) Flow cytometry analysis showed that the apoptosis in H460 and A549 cells was promoted after si-NR2F1-AS1 transfection. (D, E) Cell migration and invasion assays revealed that the migratory and invasive capacities of H460 and A549 cells were hindered by NR2F1-AS1 inhibition (x200 magnification). *P < 0.05 and **P < 0.01.