Research Paper Volume 13, Issue 5 pp 7660—7675

Long noncoding RNA NR2F1-AS1 promotes the malignancy of non-small cell lung cancer via sponging microRNA-493-5p and thereby increasing ITGB1 expression

Repression of miR-493-5p reverses the impacts of NR2F1-AS1 silencing on NSCLC cells. H460 and A549 cells were transfected with si-NR2F1-AS1 in combination with miR-493-5p inhibitor or NC inhibitor. (A, B) Rescue effects of miR-493-5p inhibition on si-NR2F1-AS1-mediated inhibition of cell growth and promotion of apoptosis in H460 and A549 cells were explored by CCK-8 assay and flow cytometry analysis. (C, D) Rescue effects of miR-493-5p inhibition on si-NR2F1-AS1-mediated suppression of cell migration and invasion in H460 and A549 cells were evaluated by cell migration and invasion assays (x200 magnification). *P

Figure 5. Repression of miR-493-5p reverses the impacts of NR2F1-AS1 silencing on NSCLC cells. H460 and A549 cells were transfected with si-NR2F1-AS1 in combination with miR-493-5p inhibitor or NC inhibitor. (A, B) Rescue effects of miR-493-5p inhibition on si-NR2F1-AS1-mediated inhibition of cell growth and promotion of apoptosis in H460 and A549 cells were explored by CCK-8 assay and flow cytometry analysis. (C, D) Rescue effects of miR-493-5p inhibition on si-NR2F1-AS1-mediated suppression of cell migration and invasion in H460 and A549 cells were evaluated by cell migration and invasion assays (x200 magnification). *P < 0.05 and **P < 0.01.