Research Paper Volume 13, Issue 5 pp 7660—7675

Long noncoding RNA NR2F1-AS1 promotes the malignancy of non-small cell lung cancer via sponging microRNA-493-5p and thereby increasing ITGB1 expression

Reintroduction of ITGB1 eliminates the functions of NR2F1-AS1 knockdown on NSCLC cells. (A) Western blotting illustrated the protein level of ITGB1 in pc-ITGB1 or pcDNA3.1-transfected H460 and A549 cells. (B, C) CCK-8 assay and flow cytometry analysis showed the proliferation and apoptosis of H460 and A549 cells after cotransfection with si-NR2F1-AS1 and pc-ITGB1 or pcDNA3.1. (D, E) Cell migration and invasion assays detected the migratory and invasive abilities of H460 and A549 cells treated as described above (x200 magnification). *P

Figure 6. Reintroduction of ITGB1 eliminates the functions of NR2F1-AS1 knockdown on NSCLC cells. (A) Western blotting illustrated the protein level of ITGB1 in pc-ITGB1 or pcDNA3.1-transfected H460 and A549 cells. (B, C) CCK-8 assay and flow cytometry analysis showed the proliferation and apoptosis of H460 and A549 cells after cotransfection with si-NR2F1-AS1 and pc-ITGB1 or pcDNA3.1. (D, E) Cell migration and invasion assays detected the migratory and invasive abilities of H460 and A549 cells treated as described above (x200 magnification). *P < 0.05 and **P < 0.01.