Research Paper Volume 13, Issue 4 pp 4962—4975

Methylation-dependent MCM6 repression induced by LINC00472 inhibits triple-negative breast cancer metastasis by disturbing the MEK/ERK signaling pathway

Overexpressed LINC00472 suppresses TNBC cell proliferation, migration and invasion. (A) The expression of LINC00472 in MDA-MB-231 cells normalized to GAPDH as determined by means of RT-qPCR. (B) MDA-MB-231 cell proliferation detected by EdU assay(× 200). (C) MDA-MB-231 cell migration detected by scratch test. (D) MDA-MB-231 cell invasion detected by Transwell assay (× 200). (E) The protein expression of N-cadherin, Vimentin, MMP9 and E-cadherin normalized to GAPDH determined by Western blot analysis. * p vs. the oe-NC group (MDA-MB-231 cells transfected with oe-NC). The results were measurement data and expressed as mean ± standard deviation. Data comparison between two groups was analyzed by independent sample t-test. The experiment was conducted 3 times independently.

Figure 2. Overexpressed LINC00472 suppresses TNBC cell proliferation, migration and invasion. (A) The expression of LINC00472 in MDA-MB-231 cells normalized to GAPDH as determined by means of RT-qPCR. (B) MDA-MB-231 cell proliferation detected by EdU assay(× 200). (C) MDA-MB-231 cell migration detected by scratch test. (D) MDA-MB-231 cell invasion detected by Transwell assay (× 200). (E) The protein expression of N-cadherin, Vimentin, MMP9 and E-cadherin normalized to GAPDH determined by Western blot analysis. * p < 0.05 vs. the oe-NC group (MDA-MB-231 cells transfected with oe-NC). The results were measurement data and expressed as mean ± standard deviation. Data comparison between two groups was analyzed by independent sample t-test. The experiment was conducted 3 times independently.