Research Paper Volume 13, Issue 4 pp 4962—4975

Figure 5. Overexpression of MCM6 activated MEK/ERK signaling pathway and restored the proliferation, migration and invasion of TNBC cells inhibited by LINC00472. (A) The expression of LINC00472 in the MDA-MB-231 cells normalized to GAPDH as determined by means of RT-qPCR. (B) The expression of MCM6 in MDA-MB-231 cells normalized to GAPDH determined by means of Western blot analysis. (C) MDA-MB-231 cell proliferation detected by EdU assay (× 200). (D) MDA-MB-231 cell migration detected by scratch test. (E) MDA-MB-231 cell invasion detected by Transwell assay (× 200). (F) The protein expression of N-cadherin, Vimentin, MMP9, E-cadherin, p-ERK, ERK, p-MEK and MEK normalized to GAPDH determined by means of Western blot analysis. * p < 0.05 vs. the oe-NC group (MDA-MB-231 cells treated with oe-NC). # p < 0.05 vs. the oe-LINC00472 group (MDA-MB-231 cells treated with oe-LINC00472). The results were measurement data and expressed as mean ± standard deviation. Data comparison among multiple groups was analyzed by one-way ANOVA followed by the Tukey’s post hoc test. The experiment was conducted 3 times independently.