Research Paper Volume 13, Issue 4 pp 4962—4975

Methylation-dependent MCM6 repression induced by LINC00472 inhibits triple-negative breast cancer metastasis by disturbing the MEK/ERK signaling pathway

Overexpression of LINC00472 inhibited tumor growth and metastasis to lungs in vivo through downregulation of MCM6. (A) The tumor volume of nude mice (n = 12). (B) The representative images of resected tumors and the tumor weights (n = 12). (C) The representative images and number of metastasis nodules in the mice (n = 12). (D) The pathological changes in lung tissues identified by means of HE staining (× 100). * p vs. the oe-NC group (nude mice bearing MDA-MB-231 cells treated with oe-NC). # p vs. the oe-LINC00472 group (nude mice bearing MDA-MB-231 cells treated with oe-LINC00472). The results were measurement data and expressed as mean ± standard deviation. Data comparison among multiple groups was analyzed by one-way ANOVA followed by the Tukey’s post hoc test. Data comparison at different time points was analyzed by repeated measures ANOVA, followed by Bonferroni’s post hoc test. n = 12. The experiment was conducted 3 times independently.

Figure 6. Overexpression of LINC00472 inhibited tumor growth and metastasis to lungs in vivo through downregulation of MCM6. (A) The tumor volume of nude mice (n = 12). (B) The representative images of resected tumors and the tumor weights (n = 12). (C) The representative images and number of metastasis nodules in the mice (n = 12). (D) The pathological changes in lung tissues identified by means of HE staining (× 100). * p < 0.05 vs. the oe-NC group (nude mice bearing MDA-MB-231 cells treated with oe-NC). # p < 0.05 vs. the oe-LINC00472 group (nude mice bearing MDA-MB-231 cells treated with oe-LINC00472). The results were measurement data and expressed as mean ± standard deviation. Data comparison among multiple groups was analyzed by one-way ANOVA followed by the Tukey’s post hoc test. Data comparison at different time points was analyzed by repeated measures ANOVA, followed by Bonferroni’s post hoc test. n = 12. The experiment was conducted 3 times independently.