Research Paper Volume 12, Issue 16 pp 16126—16141

SIRT1 alleviates high-magnitude compression-induced senescence in nucleus pulposus cells via PINK1-dependent mitophagy

Figure 3. Measurement of ROS content and mitochondrial membrane potential in the NP cells with or without Ad-SIRT1 treatment under high-magnitude compression. (A) After high-compression or high-compression plus Ad-SIRT1 treatment, the ROS level in NP cells was observed and measured by fluorescence microscopy (200×) and flow cytometry following DCFH-DA fluorescent probe staining. (B) The mitochondrial membrane potential of NP cells was observed and measured by fluorescence microscopy (200×) and flow cytometry after JC-1 fluorescent probe staining. The difference in mitochondrial membrane potential is presented by the green/red fluorescence intensity ratio. *P<0.05 versus the control group. #P<0.05 versus the high-compression group. White bars=100 μm.