Figure 3. Insulin-induced activation of AKT/S6 cascade in metabolically active tissues of GHRH-/- mice. (A–C) Activation of AKT and S6 in liver. (D–F) Activation of AKT and S6 in skeletal muscle. (G–I) Activation of AKT and S6 in subcutaneous white adipose tissue. (J–L) Activation of AKT and S6 in visceral white adipose tissue. (M–O) Activation of AKT and S6 in interscapular brown adipose tissue. The 4 hours fasted mice were injected i.p. with porcine insulin (1 IU/kg of body weight). After 20 min, tissues were collected to perform western blots. All data (means ± sem) are expressed as fold change compared to vehicle treated WT controls (defined as 1.0) (n=4 for WT group; n=6 for GHRH-/- mice). Statistical analysis was performed by unpaired Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.