Figure 2. Celastrol inhibited the adipogenic differentiation of BM-MSCs in vitro. (A, B) Representative images of Oil Red O staining of lipids (A) and quantification of the number of spots (B) on BM-MSCs cultured in the adipogenesis induction medium for 14 days. Scale bar: 100 μm. (C) qRT-PCR analysis of the relative levels of Pparg and Fabp4 mRNA expression in BM-MSCs cultured in the adipogenesis induction medium for 48 hours (n = 3 per group). Results are shown as mean ± SD. Statistical significance was determined using analysis of variance (one-way ANOVA). *P < 0.0001 compared with control.