Research Paper Volume 12, Issue 17 pp 16921—16935

Epigenetic upregulation of HOXC10 in non-small lung cancer cells

Figure 1. Expression of HOXC10 in lung adenocarcinoma cells. (A) Total cell RNA was extracted from A549 and NCI-H23 cells. The transcriptomes from A549 and NCI-H23 cells were obtained and compared using RNA-SEQ. The differentially expressed HOX genes were illustrated in heatmap. HOXC10 was marked by a red rectangle. (B) Similar to part A except that the RNA levels of HOXC10 were measured and compared between A549 and NCI-H23 cells using qRT-PCR. A fold change was obtained by normalizing to the housekeeping gene RPLP0 and setting the values from the DMSO control group to one. (C) Total protein was extracted from A549 and NCI-H23 cells. Immunoblotting was used to assess and compare the protein levels of HOXC10 between A549 and NCI-H23 cells. GAPDH was measured as a loading control. (D) Overall survival rate was compared between the patients with increase in HOXC10 expression and the rest patients in the TCGA Lung Adenocarcinoma cohort. (E) Diseases free status was compared between the patients with increase in HOXC10 expression and the rest patients in the TCGA Lung Adenocarcinoma cohort. When presented, means and standard deviations were obtained from at least 3 independent experiments. *** indicates a P value < 0.001.