RAI14 modulates proliferation, apoptosis, and cell cycle in EC. (A and B) Cell viability in cells that were transfected with the modulated lentiviruses was detected by the CCK-8 assay. TE-1 cells that were transfected with siNC, siRAI14-1, or siRAI14-2 (A); TE-14 cells that were transfected with the RAI14-vector or RAI14 (B). *P < 0.05 vs Control group. (C) Annexin V–FITC/PI staining discriminated between cells that were in early (lower right quadrant) and advanced (upper right quadrant) apoptotic states. Viable cells were double negative (lower left quadrant). (D) Cell cycle distribution after RAI14 modulation was determined. (E) The apoptosis rate (including the early and advanced apoptotic states). *P < 0.05: apoptosis rate in the modulation group versus thee respective control. (F) Proportion of cells in each cell-cycle phase. *P < 0.05: percentage of cells in S phase in the modulation group versus the respective control.