Research Paper Volume 12, Issue 18 pp 18127—18136
GHR knockdown enhances the sensitivity of HCC cells to sorafenib
Figure 1. Sorafenib induced GHR expression in HCC cell lines. (A) Western blotting assay measured the protein levels of GHR in HepG2 and Huh7 cell lines treated with sorafenib, regorafenib, lenvatinib, or cabozantinib; (B) The quantification of GHR protein expression was normalized by Image J; (C) The mRNA levels of GHR were detected in different HCC cell lines with the treatment of 0, 5 μM or 10 μM sorafenib by Real-time PCR assay. (D) Western blotting assay detected the protein levels of GHR in different HCC cell lines treated with 0, 5 μM or 10 μM sorafenib.