Research Paper Volume 13, Issue 10 pp 14469—14481

Silencing lncRNA XIST exhibits antiproliferative and proapoptotic effects on gastric cancer cells by up-regulating microRNA-132 and down-regulating PXN

PXN enhances tumor formation ability, cell proliferation and metastasis, but suppresses cell apoptosis in gastric cancer. (A) Relative expression of PXN in gastric cancer tissues (n = 65) and the adjacent normal tissues determined by RT-qPCR; *, p B) Relative expression of PXN in gastric cancer tissues from patients with metastasis (n = 45) and without metastasis (n = 20) determined by RT-qPCR; *, p C–F), SGC7901 cells were transfected with oe-PXN or co-transfected with oe-PXN and si-XIST. (C) The mRNA expression of PXN detected by RT-qPCR; (D) Cell proliferation ability in each group detected by EdU assay (× 400); (E) Cell migration ability detected by scratch test (× 40); (F) Cell apoptosis ability detected by flow cytometry; (G) The effect of PXN and lncRNA XIST on tumor formation in nude mice injected with the stably transfected SGC7901 cells. All measurement data and statistical results were expressed as mean ± standard deviation. *, p

Figure 5. PXN enhances tumor formation ability, cell proliferation and metastasis, but suppresses cell apoptosis in gastric cancer. (A) Relative expression of PXN in gastric cancer tissues (n = 65) and the adjacent normal tissues determined by RT-qPCR; *, p < 0.05 compared with adjacent normal tissues; (B) Relative expression of PXN in gastric cancer tissues from patients with metastasis (n = 45) and without metastasis (n = 20) determined by RT-qPCR; *, p < 0.05 compared with gastric cancer tissues from patients without metastasis; In the panels (CF), SGC7901 cells were transfected with oe-PXN or co-transfected with oe-PXN and si-XIST. (C) The mRNA expression of PXN detected by RT-qPCR; (D) Cell proliferation ability in each group detected by EdU assay (× 400); (E) Cell migration ability detected by scratch test (× 40); (F) Cell apoptosis ability detected by flow cytometry; (G) The effect of PXN and lncRNA XIST on tumor formation in nude mice injected with the stably transfected SGC7901 cells. All measurement data and statistical results were expressed as mean ± standard deviation. *, p < 0.05 compared with cells transfected with oe-NC or the mice injected with the oe-NC-transfected cells. Comparisons among multiple groups were analyzed using one-way ANOVA. The experiment was repeated 3 times.