Research Paper Volume 13, Issue 2 pp 1842—1858

Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma

DAPK1 regulates endoplasmic reticulum stress-mediated apoptosis. (A) GSEA shows that DAPK1 expression correlates with reactome

Figure 7. DAPK1 regulates endoplasmic reticulum stress-mediated apoptosis. (A) GSEA shows that DAPK1 expression correlates with reactome_er_phagosome_pathway and protein_localization_to_endoplasmic_reticulum pathway. (B) Western blotting shows ATF6 protein levels in control and DAPK1 knockdown ACHN-R, and control and DAPK1-overexpressing 786O-R cells. GAPDH is used as internal control. (C, D) Flow cytometry analysis shows the percentage of apoptotic cells in control and DAPK1 overexpressing 786O-R and ACHN-R cells. (E) Recovery experiment to verify the regulation of ATF6 by DAPK1.