Research Paper Volume 12, Issue 18 pp 18181—18191

Figure 2. Responses of HAECs to Ox-LDL treatment are NLRP3 dependent. Next, shNLRP3-HAECs and scr-HAECs were challenged in an in vitro AS model by treatment with 100μg/ml oxidized low-density lipoprotein (ox-LDL). (A, B) Analyses of cell apoptosis by FITC Annexin V Apoptosis assay, shown by representative flow charts (A), and by quantification (B). (C, D) Analyses of cell proliferation by Edu assay, shown by quantification (C), and by representative images (D). (E) Quantification of viable cell number in an CCK-8 assay. (F) DHE assay for ROS. *p<0.05. N=5. Scale bars are 20 μm.