Research Paper Volume 12, Issue 18 pp 18181—18191

Figure 3. Preparation of endothelia-specific NLRP3 mutant mice in ApoE-null background. (A) Endothelia-specific NLRP3 mutant mice were prepared in ApoE-null background. Tie2 is an endothelia-specific marker. Tie2p-Cre and conditional NLRP3 mutation mice were first bred to each other to generate endothelia-specific NLRP3 mutant mice Tie2p-Cre/NLRP3^MKO, and then bred to ApoE (-/-) mice to generate APOEKO/Tie2p-Cre/NLRP3^MKO mice. ApoE (-/-) mice were used as controls (APOEKO). (B) Representative genotyping. -, negative control. +, positive control.