Research Paper Volume 13, Issue 1 pp 228—240

Downregulation of miR-155-5p enhances the anti-tumor effect of cetuximab on triple-negative breast cancer cells via inducing cell apoptosis and pyroptosis

Downregulation of miR-155-5p enhances the anti-tumor effect of cetuximab in TNBC cells in vivo. EGFR-overexpressed MDA-MB-468 cells were subcutaneously injected into nude mice. (A) Tumor volumes of the mice were measured weekly. (B) The tumors were excised from xenografts and imaged on day 28. (C) The tumor weights were calculated. (D) RT-qPCR was used to determine the level of miR-155-5p in the tumor tissues. (E, F) The number of Ki67 positive cells in tumor tissues was measured using an immunohistochemistry (IHC) analysis. (G, H) The cell apoptosis in tumor tissues was measured using a TUNEL analysis. *P ##P

Figure 7. Downregulation of miR-155-5p enhances the anti-tumor effect of cetuximab in TNBC cells in vivo. EGFR-overexpressed MDA-MB-468 cells were subcutaneously injected into nude mice. (A) Tumor volumes of the mice were measured weekly. (B) The tumors were excised from xenografts and imaged on day 28. (C) The tumor weights were calculated. (D) RT-qPCR was used to determine the level of miR-155-5p in the tumor tissues. (E, F) The number of Ki67 positive cells in tumor tissues was measured using an immunohistochemistry (IHC) analysis. (G, H) The cell apoptosis in tumor tissues was measured using a TUNEL analysis. *P < 0.05, **P < 0.01 compared with the vehicle group. ##P < 0.01 compared with the cetuximab 10 nM group.