Research Paper Volume 12, Issue 17 pp 17353—17366

Taurine suppresses ROS-dependent autophagy via activating Akt/mTOR signaling pathway in calcium oxalate crystals-induced renal tubular epithelial cell injury

Figure 5. Tau alleviates oxidative injury induced by CaOx crystals. (A) The ROS level in HK-2 cells after different treatments was examined using DCFH-DA; scale bar: 50 μm. (B) Quantitative analyses of DCFH-DA assay. (C and D) Effects of Tau on SOD and MDA levels in cells treated with CaOx crystals. (E) The ultrastructural morphology of the was detected by TEM. Red arrows: mitochondria; scale bar: 500 nm. (F) Mitochondrial membrane potential of all groups of cells. (G and H) Effects of Tau on SOD and MDA levels in EG-induced renal tissues. (I) The ultrastructural morphology of mitochondria in renal tissues was detected by TEM. Red arrows: mitochondria; scale bar: 1 μm. Data are presented as the mean±SD (n=3). *P < 0.05 versus the control group, #P < 0.05 versus the CaOx group or the EG group.