Research Paper Volume 12, Issue 17 pp 17367—17379

G-protein coupled estrogen receptor activation protects the viability of hyperoxia-treated primary murine retinal microglia by reducing ER stress

Figure 6. GPER activation reduces hyperoxia-induced IPER-dependent calcium release from the ER in primary retinal microglia. (A) The bar graphs show the rates of calcium release from the ER at 12, 24, 48 and 72 h in the (a) control (C), (b) hyperoxia (H), (c) hyperoxia+DMSO (DMSO), (d) hyperoxia+G-1 (G-1), and (e) hyperoxia+G-1+G-15 (G-1+G-15) groups of primary retinal microglia. (B) The curves show the IPER-dependent calcium release rates between 30 s to 120 s from the ER for each of the five treatment groups of primary retinal microglia at 12, 24, 48 and 72 h. (C) The curves show the calcium release rates from the ER in all 5 groups of primary retinal microglia between 12-72 h. P<0.05 denotes statistical significance. The experiments were repeated thrice and means±S.D are shown.