Research Paper Volume 12, Issue 14 pp 13958—13978
Decreased autophagy and fuel switching occur in a senescent hepatic cell model system
Figure 4. Seahorse extracellular flux analysis for mitochondrial metabolic parameters and fuel oxidation in senescent AML12 cells. Agilent Seahorse XF Mito Stress Test kit was used with Agilent Seahorse XFe96 Extracellular Flux Analyzer to analyze control and senescent AML12 cell’s mitochondrial metabolic potential (A and B). (C) Agilent Seahorse XF Mito Fuel Flex kit was used with Agilent Seahorse XFe96 Extracellular Flux Analyzer to analyze control and senescent AML12 cell’s mitochondrial fuel (glucose, glutamine, and fatty acids) oxidation. All the parameters presented in the panel B and C were calculated using Seahorse Wave Desktop software. Statistical differences were calculated significant as *p<0.05 and ****p<0.0001.