Research Paper Advance Articles

Transcriptional regulation of miR-483-3p mediated by IL-6/STAT3 axis promoted epithelial-mesenchymal transition and tumor stemness in glioma

Figure 2. miR-483-3p silencing suppressed glioma cell EMT process. (A, D) qRT-PCR analysis showed efficiency of AMO-483-3p in U251 and U87 cells. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. (B, E) Western bolt analysis showed reduction of protein levels of vimentin and TWIST, E-cad and N-cad of in U251 and U87 cells. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. (C, F) Immunostaining of vimentin in U251 and U87 cells. n=4 independent cell cultures. **P<0.05.