Research Paper Volume 13, Issue 5 pp 7676—7690

Downregulation of microRNA-29b by DNMT3B decelerates chondrocyte apoptosis and the progression of osteoarthritis via PTHLH/CDK4/RUNX2 axis

DNMT3B promotes RUNX2 ubiquitination and proteasome degradation in a CDK4-dependent manner. (A) The interaction between endogenous RUNX2 and CDK4 in chondrocytes detected by Co-IP assay. (B) The expression of ubiquitinated RUNX2 protein normalized to β-actin measured by Western blot assay. (C) The degradation of RUNX 2 in chondrocytes treated with CHX. *p

Figure 5. DNMT3B promotes RUNX2 ubiquitination and proteasome degradation in a CDK4-dependent manner. (A) The interaction between endogenous RUNX2 and CDK4 in chondrocytes detected by Co-IP assay. (B) The expression of ubiquitinated RUNX2 protein normalized to β-actin measured by Western blot assay. (C) The degradation of RUNX 2 in chondrocytes treated with CHX. *p < 0.05. Statistical data were measurement data and described as the mean ± standard deviation. The repeated-measures ANOVA was applied for the comparison of data at different time points followed by Bonferroni’s post hoc test. The experiment was repeated 3 times independently.