Research Paper Volume 13, Issue 5 pp 7676—7690

Downregulation of microRNA-29b by DNMT3B decelerates chondrocyte apoptosis and the progression of osteoarthritis via PTHLH/CDK4/RUNX2 axis

DNMT3B modulates the functions of IL-1β-treated chondrocytes through the downregulation of RUNX2. IL-1β-treated chondrocytes were co-transfected with oe-DNMT3B/oe-NC and oe-RUNX2/oe-NC. (A) The viability of IL-1β-treated chondrocytes evaluated by MTT assay. (B) The apoptosis of IL-1β-treated chondrocytes assessed by flow cytometry. (C) The cell cycle distribution of IL-1β-treated chondrocytes assessed by flow cytometry. (D) Immunofluorescence staining of Ki67 and Aggrecan in IL-1β-treated chondrocytes (× 200, scale bar = 50 μm). (E) The protein expression of Ki67, Aggrecan, MMP3, MMP13 in IL-1β-treated chondrocytes normalized to β-actin measured by Western blot analysis. *p

Figure 6. DNMT3B modulates the functions of IL-1β-treated chondrocytes through the downregulation of RUNX2. IL-1β-treated chondrocytes were co-transfected with oe-DNMT3B/oe-NC and oe-RUNX2/oe-NC. (A) The viability of IL-1β-treated chondrocytes evaluated by MTT assay. (B) The apoptosis of IL-1β-treated chondrocytes assessed by flow cytometry. (C) The cell cycle distribution of IL-1β-treated chondrocytes assessed by flow cytometry. (D) Immunofluorescence staining of Ki67 and Aggrecan in IL-1β-treated chondrocytes (× 200, scale bar = 50 μm). (E) The protein expression of Ki67, Aggrecan, MMP3, MMP13 in IL-1β-treated chondrocytes normalized to β-actin measured by Western blot analysis. *p < 0.05. Statistical data were measurement data and described as the mean ± standard deviation. The one-way analysis of variance was used for comparison among multiple groups, followed by Tukey’s post hoc test. The repeated-measures ANOVA was applied for the comparison of data at different time points, followed by Bonferroni’s post hoc test. The experiment was repeated 3 times independently.