Research Paper Volume 12, Issue 14 pp 13939—13957

Both ghrelin deletion and unacylated ghrelin overexpression preserve muscles in aging mice

Figure 6. Inflammatory response in old WT, Tg, and Ghrl KO mice. (A) TNF-α (Tnf) expression in epididymal fat of 3-month old (young) and 24-month old (old) WT, Tg, and Ghrl KO mice, determined by real-time RT-PCR. Data in bar graph are presented as mean ± SEM. Young mice: WT= 3, Tg= 4, Ghrl KO = 4; old mice: WT= 5, Tg= 7, Ghrl KO = 5. (B) Serum biomarkers involved in systemic inflammation normalized on the levels in young WT mice (reference line). Young mice WT=4; old mice: WT= 4, Tg= 4, Ghrl KO = 4 pulled into two groups. Data in bar graph are presented as mean ± SD. *p<0.05 and **p<0.01, old vs. young; $p<0.05 Tg and Ghrl KO vs. WT.