Figure 4. D-gal induced premature senescence of other GBM cells. (A) A schematic illustration of D-gal-induced senescence of U87MG cells. (B) Representative images showing β-galactosidase staining in control U87MG cells and U87MG cells treated with D-gal at 222 mM for 7 and 9 d. (C) Quantification of the percentage of β-galactosidase+ U87MG cells over total cells as shown in (B) (n=15). (D) Immunostaining of PH3 (green) in control U87MG cells and senescent U87MG cells. (E) Quantitative analysis of the percentage of PH3+ cells over total cells in as shown (D) (n=15). (F) Western blot detected the expression of Lamin B1 and p53 proteins in control U87MG cells and U87MG cells treated with 222 mM D-gal for 7 d. (G, H) Quantification of Lamin B1 (n=5) and p53 (n=5) expression as shown in (F). Scale bars, 20 μm. Data shown are mean ± s.e.m. *P < 0.05, ***P < 0.001.