Figure 3. Graphene oxide (GO) inhibited metastasis in Hela cells. (A) The wound closure and wound healing rate of Hela cells treated with different doses of GO at 0, 6, 12 h and 24 h by wound healing assay. (B) Cell migration and migration rates in Hela cells treated with different doses of GO by Transwell assay. (C) Expression of metastasis-related proteins, including matrix metalloproteinase 2 (MMP2), MMP3, MMP9, intercellular adhesion molecule (ICAM), vascular cell adhesion molecule (VCAM), collagen type I (Col-1), Col-3, Racl, Rho and Cdc42, by western blotting. *P < 0.05, and **P < 0.01 vs control cells.