Research Paper Volume 12, Issue 17 pp 17634—17646

DUSP4 directly deubiquitinates and stabilizes Smad4 protein, promoting proliferation and metastasis of colorectal cancer cells

Figure 1. DUSP4 gene was highly expressed in colorectal cancer tissues. (A) mRNA abundance analysis of DUSP4 gene in GEPIA database. (B) mRNA abundance analysis of DUSP4 gene in oncomine. (C) Western blot analysis of normal tissues and colorectal cancer tissues. N: normal tissues; T: tumor tissues. GAPDH was employed as an internal reference. (D) qRT-PCR analysis of DUSP4 mRNA abundance in four colorectal cancer tissues and the paired normal tissues. (E and F) Immunohistochemical analysis of normal tissues and colorectal cancer tissues. *P<0.05. ***P<0.001.