Research Paper Volume 12, Issue 18 pp 18522—18544

GPA peptide inhibits NLRP3 inflammasome activation to ameliorate colitis through AMPK pathway

Figure 5. GPA blocked NLRP3 activation by inhibiting ROS production. THP-1 cells were primed with LPS for 4h, followed by GPA or NAC treatment 6 h before stimulation with ATP for 30 min. Levels of the ROS was measured in THP-1 cells (A). Quantitative real-time PCR analyzed of mtDNA in THP-1 cells (B). Immunoblot analyzed of IL-1β in supernatants and cell lysate of THP-1 cells (C). IL-1β in supernatants of THP-1 cells was detected by ELISA (D). Immunoblot analyzed of mitochondrial components of NLRP3 inflammasome in THP-1 cells (E). THP-1 cells were primed with LPS for 4h, followed by GPA treatment 6 h before stimulation with H2O2 for 4 h, IL-1β in supernatants of THP-1 cells was detected by ELISA (F). Data are presented as mean ± SD, three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001.