Research Paper Volume 12, Issue 18 pp 18545—18560

MiR-520d-5p modulates chondrogenesis and chondrocyte metabolism through targeting HDAC1

Figure 1. Elevated expression of miR-520d-5p and decreased level of HDAC1 in the chondrogenesis of hMSCs. (A) Volcano plot showing the differentially expressed miRNAs in hMSCs during chondrogenesis at 0 days and 21 days, as detected by RNA-seq assay. Red and green dots represent upregulated and downregulated miRNAs, respectively, according to fold change > 2 and p < 0.05. (B) Heatmap showing the expressions of differentially expressed miRNAs, as detected by qRT-PCR. (C) mRNA expression of HDAC1 in hMSCs at 0 days and 21 days of chondrogenesis. (D) Protein expression of HDAC1 in hMSCs at 0 days and 21 days of chondrogenesis. (E) mRNA expressions of the chondrogenic markers AGGRECAN, COMP, COL2A1, and SOX9 and the hypertrophic markers COL10A1 and RUNX2 at 0 days and 21 days of chondrogenesis. (F) Protein expressions of the chondrogenic markers AGGRECAN, COMP, COL2A1, and SOX9 and the hypertrophic markers COL10A1 and RUNX2 at 0 days and 21 days of chondrogenesis. For each experiment, at least three replicates were available for the analysis. Data were expressed as mean ± standard deviation (SD). *P < 0.05; ** P < 0.01; *** P < 0.001.