Research Paper Volume 12, Issue 18 pp 18545—18560

MiR-520d-5p modulates chondrogenesis and chondrocyte metabolism through targeting HDAC1

Figure 5. CI994 promotes chondrogenic differentiation of hMSCs. (A) mRNA expressions of the chondrogenic markers AGGRECAN, COMP, COL2A1, and SOX9 and the hypertrophic markers COL10A1 and RUNX2 in hMSCs treated with negative control (control), chondrogenic culture without CI994 (C-CI994), and chondrogenic culture with CI994 (C+CI994) (100 nM) at 21 days post-treatment. (B) Protein expression of COL2A1 and acetylation of histone H3 (H3_acetyl) in hMSCs treated with negative control (control), chondrogenic culture without CI994 (C-CI994), and chondrogenic culture with CI994 (C+CI994) (100 nM) at 21 days post-treatment. (C) Immunohistochemistry assay for collagen type II in hMSCs treated with negative control (control), chondrogenic culture without CI994 (C-CI994), and chondrogenic culture with CI994 (C+CI994) (100 nM) at 21 days post-treatment (16× magnification). Scale bar = 100 μm. For each experiment, at least three replicates were available for the analysis. Data were expressed as mean ± standard deviation (SD). *P < 0.05; ** P < 0.01; *** P < 0.001.