Research Paper Volume 12, Issue 18 pp 18545—18560

MiR-520d-5p modulates chondrogenesis and chondrocyte metabolism through targeting HDAC1

Figure 7. Knockdown of HDAC1 attenuates the effect of the downregulation of miR-520d-5p in PHCs. (A) mRNA expressions of HDAC1, the chondrogenic markers AGGRECAN, COMP, COL2A1, and SOX9 and the hypertrophic markers COL10A1 in PHCs treated with HDAC1 siRNA negative control (siHDAC1-NC) and HDAC1 siRNA (siHDAC1). (B) Protein expressions of HDAC1, COL2A1, and SOX9 and acetylation of histone H3 (H3_acetyl) in PHCs treated with HDAC1 siRNA negative control (siHDAC1-NC) and HDAC1 siRNA (siHDAC1). (C) mRNA expressions of HDAC1, the chondrogenic markers AGGRECAN, COMP, COL2A1, and SOX9 and the hypertrophic markers COL10A1 in PHCs treated with miR-520d-5p negative control (miR-520d-5p-NC), miR-520d-5p inhibitors (miR-520d-5p-IN), miR-520d-5p inhibitors (miR-520d-5p-IN) plus siRNA negative control (siHDAC1-NC), or miR-520d-5p inhibitors (miR-520d-5p-IN) plus HDAC1 siRNA (siHDAC1). (D) Protein expressions of HDAC1, COL2A1, and SOX9 and acetylation of histone H3 (H3_acetyl) in PHCs treated with miR-520d-5p negative control (miR-520d-5p-NC), miR-520d-5p inhibitors (miR-520d-5p-IN), miR-520d-5p inhibitors (miR-520d-5p-IN) plus siRNA negative control (siHDAC1-NC), or miR-520d-5p inhibitors (miR-520d-5p-IN) plus HDAC1 siRNA (siHDAC1). For each experiment, at least three replicates were available for the analysis. Data were expressed as mean ± standard deviation (SD). *P < 0.05; ** P < 0.01; *** P < 0.001.