Figure 8. Inhibiting PERK suppresses light-related autophagy. (A) 661W cells were infected with lentivirus-expressed PERK shRNA (sh-PERK) or negative control shRNA (NC). The level of PERK in the whole cell lysate was determined with western blotting, and β-actin was referenced as an internal control. (B) 661W cells with stable PERK knockdown/ARPE-19 cells treated with GSK (5 μM) or vehicle were cultured under light/dark conditions for 3 days. The target proteins in the whole cell lysate were determined with western blotting, and β-actin was referenced as an internal control. Three independent experiments are conducted two weeks apart. The results are presented as the mean± SEM. n (per group) =3, NS: no significance, *P < 0.05, **P < 0.01.